专利名称：Human monoclonal antibodies directed
against the transmembrane glycoprotein(gp41) of human immunodeficiency virus-1(HIV-1)
发明人：Cotropia, Joseph P.申请号：EP00102215.1申请日：19911221公开号：EP1016671A2公开日：20000705
专利附图：
摘要：A method for neutralizing HIV-1 is disclosed. A preferred embodiment utilizes a
novel human monoclonal antibody that binds to a conserved region of the gp41transmembrane subunit of the virus. The antibody is produced by continuous cell linesdeveloped using human B lymphocyte cells collected from a patient possessing hightiters of anti-HIV antibodies. The conserved region bound by the neutralizing antibody isa peptide of approximately 12 amino acids in length and preferably 8-10 amino acids.Similar regions on HIV-2 and SIV are also disclosed. The present invention is furtherdirected to a kit and a method for detecting the presence and determining concentrationof an antibody that inhibits HIV-1 fusion-associated epitope, a peptide on gp41 with theamino acid sequence represented by GCSGKLIC. The detection and quantitation methodincludes an enzyme-linked immunosorbent assay (ELISA). The determination of theconcentration of the antibody that inhibits HIV-1 fusion-associated epitope on gp41,present in body fluids of a patient seropositive for HIV-1 antibodies, provides thephysician with an objective means to form a prognosis for each individual case and assiststhe clinician therefore in determining the appropriateness to initiate medical interventionor change therapy.
申请人：Cotropia, Joseph P.
地址：813 Carpenter Street Philadelphia, Pennsylvania 19147 US
国籍：US
代理机构：UEXKÜLL & STOLBERG
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